abstract：:The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...

journal_title：Plant molecular biology

authors： Carmel-Goren L,Liu YS,Lifschitz E,Zamir D

更新日期：2003-08-01 00:00:00

abstract：:In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...

journal_title：Plant molecular biology

authors： Kanno A,Saeki H,Kameya T,Saedler H,Theissen G

更新日期：2003-07-01 00:00:00

abstract：:Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several...

journal_title：Plant molecular biology

authors： Kersten B,Feilner T,Kramer A,Wehrmeyer S,Possling A,Witt I,Zanor MI,Stracke R,Lueking A,Kreutzberger J,Lehrach H,Cahilll DJ

更新日期：2003-07-01 00:00:00

abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

journal_title：Plant molecular biology

authors： Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

更新日期：2003-06-01 00:00:00

abstract：:We isolated and characterized different classes of transposable DNA elements in oil palm (Elaeis guineensis) plants grown from seed, and plants regenerated from tissue culture that show mantling, an abnormality leading to flower abortion. Using PCR assays, reverse transcriptase fragments belonging to LINE-like and gyp...

journal_title：Plant molecular biology

authors： Kubis SE,Castilho AM,Vershinin AV,Heslop-Harrison JS

更新日期：2003-05-01 00:00:00

abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

journal_title：Plant molecular biology

authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

更新日期：2003-05-01 00:00:00

abstract：:Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...

journal_title：Plant molecular biology

authors： Asakura Y,Hagino T,Ohta Y,Aoki K,Yonekura-Sakakibara K,Deji A,Yamaya T,Sugiyama T,Sakakibara H

更新日期：2003-05-01 00:00:00

abstract：:Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...

journal_title：Plant molecular biology

authors： Chaal BK,Ishida K,Green BR

更新日期：2003-05-01 00:00:00

abstract：:In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...

journal_title：Plant molecular biology

authors： Stenzel I,Hause B,Miersch O,Kurz T,Maucher H,Weichert H,Ziegler J,Feussner I,Wasternack C

更新日期：2003-04-01 00:00:00

abstract：:Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...

journal_title：Plant molecular biology

authors： Daley DO,Considine MJ,Howell KA,Millar AH,Day DA,Whelan J

更新日期：2003-03-01 00:00:00

abstract：:HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

journal_title：Plant molecular biology

authors： Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

更新日期：2003-03-01 00:00:00

abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

journal_title：Plant molecular biology

authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

更新日期：2003-02-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

journal_title：Plant molecular biology

authors： Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

更新日期：2003-01-01 00:00:00

abstract：:TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...

journal_title：Plant molecular biology

authors： Chen S,Peng S,Huang G,Wu K,Fu X,Chen Z

更新日期：2003-01-01 00:00:00

abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...

journal_title：Plant molecular biology

authors： Pimpl P,Denecke J

更新日期：2002-12-01 00:00:00

abstract：:14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...

journal_title：Plant molecular biology

authors： Roberts MR,Salinas J,Collinge DB

更新日期：2002-12-01 00:00:00

abstract：:A cDNA coding for phytocystatin, a protease inhibitor, was isolated from wheat embryos by differential display RT-PCR and the corresponding full-length cDNA (named WC5 for wheat cystatin gene 5) subsequently obtained by RACE. The deduced primary sequence of the protein suggests the presence of a 28 amino acid N-termin...

journal_title：Plant molecular biology

authors： Corr-Menguy F,Cejudo FJ,Mazubert C,Vidal J,Lelandais-Brière C,Torres G,Rode A,Hartmann C

更新日期：2002-11-01 00:00:00

abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

journal_title：Plant molecular biology

authors： Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

更新日期：2002-10-01 00:00:00

abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...

journal_title：Plant molecular biology

authors： Majira A,Domin M,Grandjean O,Gofron K,Houba-Hérin N

更新日期：2002-10-01 00:00:00

abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...

journal_title：Plant molecular biology

authors： de la Paz Sánchez M,Torres A,Boniotti MB,Gutierrez C,Vázquez-Ramo JM

更新日期：2002-09-01 00:00:00

abstract：:We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...

journal_title：Plant molecular biology

authors： Fung KL,Zhao KJ,He ZM,Chye ML

更新日期：2002-09-01 00:00:00

abstract：:Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

journal_title：Plant molecular biology

authors： Osakabe K,Yoshioka T,Ichikawa H,Toki S

更新日期：2002-09-01 00:00:00

abstract：:The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

journal_title：Plant molecular biology

authors： Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

更新日期：2002-08-01 00:00:00

abstract：:During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

journal_title：Plant molecular biology

authors： Ngo P,Ozga JA,Reinecke DM

更新日期：2002-07-01 00:00:00

abstract：:Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...

journal_title：Plant molecular biology

authors： Scherer GF

更新日期：2002-06-01 00:00:00

abstract：:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

journal_title：Plant molecular biology

authors： Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

更新日期：2002-05-01 00:00:00

abstract：:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

journal_title：Plant molecular biology

authors： Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

更新日期：2002-05-01 00:00:00

abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...

journal_title：Plant molecular biology

authors： Menz MA,Klein RR,Mullet JE,Obert JA,Unruh NC,Klein PE

更新日期：2002-03-01 00:00:00

abstract：:The variation in grain hardness is the single most important trait that determines end-use quality of wheat. Grain texture classification is based primarily on either the resistance of kernels to crushing or the particle size distribution of ground grain or flour. Recently, the molecular genetic basis of grain hardnes...

journal_title：Plant molecular biology

authors： Morris CF

更新日期：2002-03-01 00:00:00

abstract：:Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...

journal_title：Plant molecular biology

authors： Sandhu D,Gill KS

更新日期：2002-03-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

journal_title：Plant molecular biology

authors： Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

更新日期：2002-02-01 00:00:00

abstract：:Angiosperm genomes show tremendous variability in genome size and chromosome number. Nevertheless, comparative genetic mapping has revealed genome collinearity of closely related species. Sequence-based comparisons were used to assess the conservation of gene arrangements. Numerous small rearrangements, insertions/del...

journal_title：Plant molecular biology

authors： Schmidt R

更新日期：2002-01-01 00:00:00

abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

journal_title：Plant molecular biology

authors： García-Ortiz MV,Ariza RR,Roldán-Arjona T

更新日期：2001-12-01 00:00:00

abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

journal_title：Plant molecular biology

authors： de Jager SM,Menges M,Bauer UM,Murra JA

更新日期：2001-11-01 00:00:00

abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

journal_title：Plant molecular biology

authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

更新日期：2001-11-01 00:00:00

abstract：:Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...

journal_title：Plant molecular biology

authors： Cheng Z,Snustad DP,Carter JV

更新日期：2001-10-01 00:00:00

abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...

journal_title：Plant molecular biology

authors： Gaspar Y,Johnson KL,McKenna JA,Bacic A,Schultz CJ

更新日期：2001-09-01 00:00:00

abstract：:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...

journal_title：Plant molecular biology

authors： Henrissat B,Coutinho PM,Davies GJ

更新日期：2001-09-01 00:00:00

abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

journal_title：Plant molecular biology

authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

更新日期：2001-08-01 00:00:00